| # | Title | Artist | Rating | Length |
|---|
| 1 | I’m a Man of Constant Sorrow | The Stanley Brothers | | 2:58 |
| 2 | Keep on the Sunny Side | The Carter Family | 5 | 2:50 |
| 3 | Big Rock Candy Mountains | Harry “Haywire Mac” McClintock | 3.35 | 2:17 |
| 4 | Hard Time Killin’ Floor Blues | Skip James | | 2:49 |
| 5 | You Are My Sunshine | Jimmie Davis | | 2:48 |
| 6 | I'll Fly Away | The Humbard Family | | 2:36 |
| 7 | I’m Blue, I’m Lonesome (Blue and Lonesome) | Bill Monroe | | 2:50 |
| 8 | Lonesome Valley | Sanctified Group | | 3:22 |
| 9 | Indian War Whoop | Hoyt Ming & His Pep-Steppers | | 3:11 |
| 10 | In the Jailhouse Now | Jimmie Rodgers | 4 | 3:19 |
| 11 | In the Highways | The Carter Family | | 1:17 |
| 12 | Sandy Land | Fiddlin' Sam Long | | 2:39 |
| 13 | John the Revelator | Blind Willie Johnson | 4 | 3:19 |
| 14 | Way Down the Old Plank Road | Uncle Dave Macon | | 3:00 |
| 15 | Country Blues | Dock Boggs | 3 | 2:59 |
| 16 | O Death | Charlie Monroe's Boys | | 2:02 |